15 October 2017: All members of the Baumann lab – Hannes, Emma, Chris, Julie and Jake had fun at an Open House event at the Avery Point Campus as part of the 50th Anniversary celebrations. The whole department participated with a flurry of educational activities and fun exhibitions.
Our lab manned a table outside the Rankin Lab, telling people about the nearshore fish community, the phenomenon of ocean acidification and the measurement of pH in water. Everybody chipped in, thanks!
Hannes also premiered reciting Dr. Seuss’ “The Lorax” in front of young and old in the AP auditorium.
Check out some of the fun around the “Ocean Acidification and our fish” table:
10 October 2017. Today, Chris, Emma, and Julie measured over 400 juvenile Atlantic silversides for their length and weight. This time, however, we did not euthanize the fish before, but successfully measured them while still alive, only a little drowsy from the mild anesthetic we administered before.
Click on the video below to have a look for yourself.
Congratulations all, for a job well done!
October, 2nd, 2017: Happy to announce that Estuaries and Coasts just published (online) our article that looked unifying principles of pH and DO fluctuations across many US nearshore habitats. The datasets belongs to the US Nearshore Estuaries Research Reserves System (NERRS) and is one of the most extensive monitoring datasets in coastal aquatic habitats. In this case, we used 15 years of continuous monitoring data (> 5 million data points) from 16 different reserves across the US Atlantic, Caribbean, Gulf of Mexico, and Pacific coasts and analyses short- to long-term variability in pH and DO fluctuations. Among the highlights:
Our analyses confirmed that large, metabolically driven fluctuations of pH and DO are a unifying feature of nearshore habitats. Even more so, we were able to show that across habitats, one can predict mean pH or mean diel pH fluctuations simply based on salinity and oxygen levels/fluctuations. This provides strong empirical evidence that common metabolic principles drive diel to seasonal pH/DO variations within as well as across a diversity of estuarine environments.
As expected, there are no monotonic trends in nearshore pH conditions, but interannual fluctuations that are of similar magnitude than the pH decrease predicted for the average surface ocean over the next three centuries.
By correlating weekly anomalies of pH, oxygen, and temperature, we found strong empirical support to the notion that coastal acidification — in addition to being driven by eutrophication and atmospheric CO2 increases — is exacerbated simply by warming, likely via increasing community respiration.
From 11-16 July, Hannes, Chris, Jake (Baumann lab, UConn) and Teresa (Nye lab, Stony Brook) were presenting research from our common NSF project at the 41st Larval Fish Conference, organized by the Early Life History Section of the American Fisheries Society in Austin, TX.
Holding the fort and maintaining experiments at Avery Point were James, Julie, and Elle. Thank you for helping out.
We gave four talks in two sessions:
Baumann H., Snyder, J.T., and Murray, C.S. 2017. Quantifying offspring CO2-sensitivity in a fish: a meta-analysis.
Snyder, J.T., Murray, C.S., and Baumann H. 2017.
Potential for maternal effects on offspring CO2 sensitivity in a coastal marine fish
Murray, C.S., Snyder, J.T., and Baumann H. 2017. A multi-factorial evaluation of temperature-dependent CO2-effects in a coastal forage fish.
Schwemmer, T., Baumann H., and Nye, J. 2017.
Physiological effects of increased temperature and carbon dioxide on Atlantic silverside early life stages <
Here is how Jake rates his first international conference experience:
Austin Texas, July 2017. “Attending the Joint Meeting of Ichthyologists and Herpetologists was my first visit to Austin Texas and my first large-conference presentation. My presentation was part of the Larval Fish Conference, a sub-section of the larger meeting, and I quickly learned how welcoming the larval fish group of researchers, scientists, professionals, and students were. Having not been to a “destination” conference like this before, I had little expectations, but I had a lot of fun networking, discussing research, and socializing. I think the coolest non-conference related event was seeing the Mexican Free-Tailed bats that live in the Congress Street Bridge, as every night around sunset they leave to go feed. Seeing hundreds of thousands of bats stream out of the bridge was incredible, and something I’d highly recommend. The city of Austin was great, and I spent much of the first day (pre-conference) exploring the city in the scorching heat. Overall the Baumann Lab had an excellent time at the conference, and can’t wait for the next one!”
It’s the beginning of June, and in the Baumann lab that means: high time for experimental research on the Atlantic Silverside, the famous forage fish and important model species! This year, we have several major objectives; our NSF-sponsored research examines the sensitivity of offspring to the individual and combined effects of high CO2 and low oxygen (Chris Murray), while in collaboration with our colleagues from Cornell University we rear several families for genetic and transcriptomic studies. Elle Parks, our REU student just started her work on the effects of CO2 and temperature on the starvation resistance of silverside larvae. As always, the days when new experiments start are a group effort, where everybody including many volunteers help. Thanks to Peter Morenus (UConn) for the coming down for documenting the activities!
The Baumann lab, June 2017: from left to right; Isaiah Mayo, Julie Pringle, Chris Murray, Elle Parks, Hannes Baumann, Jacob Snyder, James Harrington + "Bear". (Photo: Peter Morenus, UConn)
Screens with enumerated embryos are suspended in each rearing container using fishing line. (Photo: Peter Morenus, UConn)
All adult silversides used to produce new offspring are getting measured and preserved. (Photo: Peter Morenus, UConn)
On 9 June, Elle and Julie strip-spawn Atlantic silverside females into spawning dishes covered in window screen for eggs to attach. (Photo: Peter Morenus, UConn)
Hydrated, ready to be fertilized eggs extrude from a running ripe female Atlantic silverside when putting gentle pressure on the abdomen. (Photo: Peter Morenus, UConn)
Chris Murray inspects a screen with newly fertilized Atlantic Silverside embryos, prior to starting a new set of experiments. (Photo: Peter Morenus, UConn)
Hannes shows Elle Parks (REU 2017), how individual screen with enumerated embryos are suspended into the replicate rearing containers. (Photo: Peter Morenus, UConn)
On 9 June 2017, members of the Baumann lab all help to start a new set of experiments in the Rankin Lab at UConn Avery Point. From left to right: Julie Pringle, Hannes Baumann, Elle Parks, Jacob Snyder, James Harrington, Isaiah Mayo, Chris Murray). (Photo: Peter Morenus, UConn)
Chris and Jake strip-spawning. (Photo: Peter Morenus, UConn)
Somewhere after Richmond, VA, the sun sets and traffic on the I-95 begins moving better. At long last. The four people in the burgundy Dogde Challenger have all already cycled through their driving shifts once and dare an impatient glance at the time left. Still more than 8 hours. More than 8 hours to reach this very special location at the Atlantic coast – Jekyll Island, Georgia. In the trunk of the car a jumble of coolers and a beach seine, buckets, air pumps, and hoses topped with the crumpled witnesses of roadside dining. This is no ordinary road trip.
We, that are Aryn and Nicholas from the Therkildsen lab of Conservation Genetics lab at Cornell University and James and Hannes from the Fish Ecology Lab here at UConn; we went on this road trip to catch live, spawning ripe Atlantic silversides from the southern edge of the species distribution. We then intended to bring these fish back to UConn alive, sample another population from the south shore of Long Island (Patchogue, NY) and produce genetic crosses of these populations.
The broad goal of our expanding collaborative efforts with our geneticist friends from Cornell is the creation of an annotated genome of this species, which will be an important milestone in deepening or understanding of the molecular and genetic responses of organisms to local selection regimes and marine climate change. Given the Atlantic silverside’s ecological importance as an abundant forage fish along the American east coast and it’s rich history as a model organism in evolutionary and ecological studies, the annotated genome is the next logical step.
Even at hindsight, the plan still seems a little insane. But it worked. We indeed managed to catch spawning silversides at the Georgia site and then transported them immediately back to our Rankin Lab, which involved another 17 hours of driving back. After securing samples from Patchogue, we indeed managed to cross single parents from each site to produce full-sib crosses that will later be used to produce what geneticist call a linkage map. Other across and within-population crosses will be used to study gene expression at two different temperatures or raise adults for producing an F2 generation.
The silverside larvae are currently well, feeding, and growing up nicely. We all cross fingers for this enterprise to end in good samples and a step forward for genetic studies on a marine fish.
The Jekyll crew Hannes, James, Nicholas, and Aryn (from left to right)
James Harrington carefully replacing water in the transport coolers for the fish. All fish survived the transport.
Andrew's Beach on Jekyll Island, GA, on 11 May 2017.
On the morning of 11 May 2017, our old beach seine is drying on the beach park of Jekyll Island, GA. After its last successful trip, the 9 year old gear has now been retired.
A newly hatched silverside larvae with a twist. It's a haploid specimen, produced by fertilizing eggs with UV-treated sperm.
The daunting view of the road trip on 10 - 12 May 2017. 34h of driving and way too little sleep in just two days.
Adult silversides from Jekyll Island, GA, swimming in our holding tanks in the Rankin Lab at UConn
NOAA sanctuaries just published a little blurb online, introducing sand lance and it’s importance to the Stellwagen Bank National Marine Sanctuary, including a small section on the current research efforts funded by NOAA Regional SeaGrant.
“To that end, the team is collaborating with scientists from the University of Connecticut (UConn). UConn study members transport live-caught sanctuary sand lance to their lab, where further generations of sand lance are raised. The resulting larval sand lance are raised in high-tech rearing facilities that can be adjusted to mimic future ocean conditions.”
4th time’s the charm: sampling spawning ripe sand lance on Stellwagen Bank
Early morning on 2 December 2016, we left Scituate, MA, for the forth time this year, heading towards Stellwagen Bank in search of spawning ripe Northern sand lance (Ammodytes dubius), a winter spawning forage fish of great importance to the Stellwagen Bank National Marine Sanctuary and the object of latest research efforts. While during the last three cruises in late October and November, we saw a progression of ripening in the specimens, up to now we didn’t actually find spawning ripe individuals. Today, though, things are different, and when the first sand lance appear in our beam trawl, we immediately know that today we’ve been at the right time and at the right place.
It seemed an ambitious dream not too long ago, but now we’re happy report that we’ve started an experiment on sand lance embryos in our lab. Thanks to Chris Murray, David Wiley, Mike Thompson, captain Steve and his deckhand Matt for the successful trip!
Check out some footage of the trip and the beam trawl operation on board of captain Steve’s fishing vessel
On 27 October 2016, Hannes, Chris and Julie joined researchers from the Stellwagen Bank National Marine Sanctuary (David Wiley, Anne-Marie Runfola, Brad Cabe, Michael Thompson), the USGS (Page Valentine, Dann Blackwood) and the crew of the R/V Auk (Dave Slocum, James Stasinos) to embark on our first of five total sampling missions in this enigmatic marine habitat. Our goal, catching live Northern sand lance, Ammodytes dubius, the so critical forage fish species that is referred to as the “backbone of the sanctuary”, because all kinds of marine predators from whales to tuna to seabirds gather on the bank to feast on them.
Our renewed efforts are part of our recently funded NOAA Regional SeaGrant Project to investigate the effects of ocean warming, acidification and low oxygen on sand lance early life stages.
As before, we first started by deploying a Seaboss sediment grab, which allows our colleagues from the USGS to characterize sediment types in association with the occurrence of sand lance. In addition, however, we brought a small beam trawl along for the first time to find out, whether we could more effectively catch sand lance and then transport them live to our seawater facility at UConn Avery Point. We are happy report that the efforts by all have paid off and that there are now ~ 180 adult ripening sand lance swimming in our tanks. Thanks all, see you again for the second survey in a few weeks!
Check out the video below, made from clips of no less than five different GoPro’s (if you listen carefully, around 2:40 into the clip you’ll hear the singing of some nearby humpback whales):
Early mornings, long days, lots of sorting and measuring, short breaks for food, and almost no time to sit for much of the day. That’s science, and that’s exactly what it was like aboard the R/V Gloria Michelle during my four-day stint. Our original hope was to sample Cape Cod Bay and the backside of the Cape, but due to weather concerns we ended up in Cape Cod bay for two days, and then the third was in Buzzards Bay and the Fourth in Outer Vineyard Sound. Why was I aboard this research vessel? Twice each year the State of Massachusetts sends out surveyors and volunteers to sample the benthic fish and invertebrate population. They sample multiple depth strata, and the entirety of Massachusetts coastal waters (by way of a sampling grid). Our lab was particularly hopeful of getting Sandlance (Ammodytes dubius), which I would have sampled for GSI and histology. Sadly, since we didn’t make it out to the backside of the Cape we saw no Sandlance, but we did see plenty of cool fish! Some highlights? A spiny dogfish (my first on this coast), a 4.5’ smooth dogfish, a 48cm Striped Sea Robin (the largest I’ve ever seen!), a few Red Cornetfish, a handful of Atlantic Moonfish, and a few dwarf goat fish. I got a crash course in otolith removal from Haddock, Winter Flounder, Fluke, and Kingfish, as well as learned how to ID many fish I’ve never seen before. It was a wonderful trip, and something I highly recommend every Biological Oceanographer (or fish biologist) volunteer for!
On 16 September 2016, Jake measures skates on board the R/V Gloria Michelle
On 15 September 2016, the bottom trawl net is emptied onto the R/V Gloria Michelle for the crew to sort, weigh and measure the catch
The R/V Gloria Michelle before leaving port on 14 September for the 2nd leg of the fall 2016 Massachusetts Bottom Trawl survey