Fishery Bulletin publishes black sea bass diet metabarcoding study

22 November 2024. We are happy to share that our paper on black sea bass stomach content metabarcoding has been published today in the traditional NOAA journal Fishery Bulletin. Our study used black sea bass juveniles caught in Mumford Cove to study their diet via a molecular approach known as metabarcoding. This method often detects rare or soft-bodied prey better than traditional morphological content analyses. We found that small, newly settled black sea bass eat mostly shrimp, but also many softbodied polychaetes. And weirdly, they seem to like one particular kind of (invasive) amphipod. Only larger juveniles seem to add fish to their diet.

Our study is a great first collaboration between our departments genomic experts (Ann Bucklin, Paola Batta-Lona) and the Evolutionary Fish Ecology Lab. The first product of our collaborative efforts has seen the light!

Fishery Bulletin is the 143 years old peer-reviewed journal managed and published by the National Marine Fisheries Service (NMFS) of the National Ocean and Atmospheric Administration (NOAA). It publishes Open Access at no costs to authors. Click the link below to download the paper.



BSB-COI-summary-web
Composition of prey detected in samples from stomachs of juvenile black sea bass (Centropristis striata), collected in Mumford Cove off Connecticut in August 2020, through metabarcoding with (A) the mitochondrial cytochrome c oxidase subunit I (COI) gene region and (B) the V9 hypervariable region of 18S rRNA. Bars represent the total number of COI and V9 gene sequences identified for each of 6 and 10 major prey taxa in DNA samples from 35 and 99 stomachs, respectively. In panel A, lists / pictures of the major prey species and their relative proportions for each prey taxon are provided (modified after Figure 1 in Batta-Lona et al. 2025)