NCMA2022 experiment update: first genetic material sampled
Posted on by Hannes Baumann
Research News
NCMA2022 experiment update: first genetic material sampled
MEPS just published our most recent paper on sand lance CO2-sensitivity!
A potential ripple effect from carbon in the atmosphere could have severe impacts throughout the ocean ecosystem
By Elaina Hancock. Reposted from UConn Today, 7 April 2022
When carbon is emitted into the atmosphere, about a quarter of it is absorbed by the earth’s oceans. As the oceans serve as a massive ‘sink’ for carbon, there are changes to the water’s pH – a measure of how acidic or basic water is. As oceans absorb carbon, their water becomes more acidic, a process called ocean acidification (OA). For years, researchers have worked to understand what effect this could have on marine life.
While most research so far shows that fish are fairly resilient to OA, new research from UConn, the University of Washington, the National Oceanic and Atmospheric Administration (NOAA), and Southern Connecticut State University, shows that an important forage fish for the Northwest Atlantic called sand lance is very sensitive to OA, and that this could have considerable ecosystem impacts by 2100. The team’s findings have just been published in Marine Ecology Progress Series 687.
Sand lance spawn in the winter months in offshore environments that tend to have stable, low levels of CO2, explains UConn Department of Marine Sciences researcher and lead author Hannes Baumann.
“Marine organisms are not living in a uniform ocean,” Baumann says. “In near shore environments, large CO2 fluctuations between day and night and between seasons are the norm, and the fish and other organisms are adapted to this variability. When we stumbled upon sand lances we suspected they are different. We thought that a fish that lives in a more open-ocean offshore environment might be more sensitive than the near-shore fish because there’s just much less variability.”
The project was a collaboration with physical oceanographers, including Assistant Professor of Marine Sciences Samantha Siedlecki and Michael Alexander from NOAA’s Physical Sciences Laboratory in Boulder, Colorado, who modeled CO2 levels in 2050 and 2100 for a specific part of the Gulf of Maine where sand lance spawn. Then Baumann and his team reared sand lance embryos in the lab under experimentally higher CO2 levels matching the projected levels.
There are instances of direct fish mortality as result of elevated CO2, but they are rare, says Baumann. However, sand lance embryos proved to be exceptionally sensitive, and fewer embryos hatched under future oceanic CO2 conditions. The researchers repeated the experiments three more times to avoid jumping to conclusions but each time they observed the same result.
“We found that embryo survival-to-hatch decreased sharply with increasing CO2 levels in the water, concluding that this is one of the most CO2-sensitive fish species studied thus far,” Baumann says.
Sand lances are surely one of the most important forage fish here on the Northwest Atlantic shelf… The humpback whales, sharks, tuna, cod, shearwaters, terns — you name it — they are all relying on sand lance.
With this interdisciplinary approach combining model forecasts and serial experimentation the researchers arrived at a picture that is much more specific.
“We consequently applied principles of serial experimentation, which is a most timely and important topic in ocean acidification research right now,” Baumann says. “Because our findings are backed up by repeated independent evidence, they are more robust than many published ocean acidification studies to date.”
In addition to preventing many sand lance embryos from developing normally, the researchers document a second negative, and novel, response to elevated CO2. Higher CO2 levels appear to make it harder for embryos to hatch.
Baumann explains the lowered pH likely renders enzymes needed for successful hatching less effective, leaving the embryos unable to break through their eggshell (chorion) to hatch.
The results show that by 2100, due to acidification, sand lance hatching success could be reduced to 71% of today’s levels. Since sand lance are such a critical component of the food web of the Northwest Atlantic, this marked decrease in sand lance would have profound impacts throughout the ecosystem.
“Sand lances are surely one of the most important forage fish here on the Northwest Atlantic shelf,” Baumann says. “Their range spans from the Mid Atlantic Bight all the way to Greenland. Where we studied them, on Stellwagen Bank, they are called the backbone of the ecosystem. The humpback whales, sharks, tuna, cod, shearwaters, terns — you name it — they are all relying on sand lance, and if sand lance productivity goes down, we will see ripple effects to all these higher trophic animals. Even though we humans don’t fish for sand lance, we need to take care of the species because it has such a huge effect on everything else.”
Baumann says this study supports the hypothesis that offshore, high latitude marine organisms like the sand lance may be among the most vulnerable to OA. As a result, these organisms and food webs will likely be impacted first and soon, and we must act now.
Previous research has focused on opportunistically chosen species when testing their sensitivity for ocean acidification, says Baumann, but this should change.
“We need strategic thinking about what species we are testing next, because we cannot test every marine fish species, that’s an impossible task. We should concentrate on fish species that are likely the most vulnerable, and therefore the ones that are probably being affected first and this research makes a compelling argument that those are the fish species at higher latitudes and in more offshore than nearshore environments.”
- Baumann, H., Jones, L.F.*, Murray, C.S., Siedlecki, S.A., Alexander, M., and Cross, E.L. (2022)
Impaired hatching exacerbates the high CO2 sensitivity of embryonic sand lance, Ammodytes dubius
Marine Ecology Progress Series 687:147-162
Kelli Mosca presents Master thesis research on Atlantic Sturgeon
Hannes gives DMS Friday seminar on sand lance ecology
Lucas Jones presents his Masters Thesis research!
ICES Journal of Marine Science publishes long-term fecundity study!
A day on the water with CTDEEPs sturgeon researchers!
28 September 2021. The day started nice enough but soon turned into dark threatening clouds, which the sturgeon researchers that day eyed with concern. Tom Savoy, Deb Pacileo, and Jacque Benway from CTDEEP, along with Kelli Mosca, Steve, Jake, and Hannes started to cast off late morning and slowly motored up the Connecticut River. "We're trying to catch the slack tide to set our sturgeon gill nets" explained Tom, the veteran sturgeon researcher, who has accompanied and steered most monitoring and protection efforts of these iconic fish over the past decades. It was Tom and his colleagues, who in 2014 caught the first baby Atlantic sturgeon in Connecticut River - a potential sign for a long hoped for recovery and the starting point of our project funded by CT SeaGrant.
The day trip was almost over, before it began. The downpour short but relentless and Jacque eyeing the lightening coming from the west with unease. But just before we could decide to fully head back, the radio call from the other boat that several sturgeon had indeed been caught in the gill nets! We therefore proceeded going through the routine measurement protocol that has been implemented for many years and is part of a federal permit to study this endangered species (No. 19641). Even a photograph is considered a sample, which is why we were glad to have our photographer Jacob Snyder (RedSkiesPhotography.com) fully accredited for the trip today. We saw with our own eyes, how a caught sturgeon was first being carefully pried loose from the gill-netting and placed in an aerated observation sump to prevent stress. All individuals are then checked for previously inserted tags using a specialized scanner (1) and if none is found, a new PIT-tag is inserted under the skin with the help of a syringe (3). All sturgeon are also measured for length (2), gape (5) and head width, and weighed (6) before being released back into the water. Tissue samples and in a limited number of fish also fin spine samples are taken for genetic information and growth data, the latter Kelli analyzed for her Masters Thesis.
The potential re-emergence of Atlantic sturgeon spawning in the Connecticut River is a success story of research and conservation, however, these efforts need to be sustained and widely communicated for the success to endure.
Nature Climate Change publishes 25 generation copepod adaptation study!
Reposted from UConn Today | August 26, 2021 | By Elaina Hancock
The world’s oceans are becoming increasingly stressful places for marine life, and experts are working to understand what this means for the future. From rising temperatures; to acidification as more carbon enters the waters; to changes in the currents; the challenges are multifaceted, making experiments and projections difficult.
Copepods are small marine animals that are abundant, widely dispersed, and serve as major structural components of the ocean’s food web. A team of scientists from the University of Connecticut, Jinan University in China, and the University of Vermont have found that a species of copepod called Acartia tonsa can cope with climate change, but at a price. Their research was just published in Nature Climate Change.
“We have this problem of climate change and in the ocean, it is a multi-dimensional problem because it’s not just the warming, the ocean is becoming more acidic where pH is going down as we pump more CO2, into the atmosphere. Organisms need to cope, they are under more stress, and things are happening very fast,” says Hans Dam, UConn professor of Marine Sciences.
Dam explains that previous studies suggest some animals will be more sensitive than others to changes like shifts in pH. Prior studies with copepods showed they are not particularly sensitive to pH changes, but Dam points out those studies were only done with a single generation, or few generations, to a single stressor and shows the ability to acclimate rather than adapt. This new study not only looks at adaptation across 25 generations, it also considered both ocean warming and acidification (OWA), something that few studies have done until now.
“If you want to study the long-term effects, you must consider the fact that animals will adapt to changes or stress in the environment, but to do that you have to do the right experiments. Most people do not do those experiments with animals because it takes a long time to study in multiple generations.”
The researchers looked at fitness, or the ability of a population to reproduce itself in one generation, and how fitness would change through generations in increased OWA conditions. The first generation exposed to new OWA conditions suffered extreme reductions of over 50% of population, says Dam. It was as if OWA was a big hammer that greatly reduced the population fitness. By the third generation, the population seemed to have mostly recovered. However, by the 12th generation, the researchers began to see declines once again.
Though the copepods were able to adapt, the adaptation was limited because fitness was never fully recovered, and the researchers suspect there are some antagonistic interactions at play, leading to a tug of war situation between adaptation to warming and to acidification. These antagonistic interactions complicate predicting what responses can be expected.
James deMayo, co-author and UConn Ph.D. student adds, “Perhaps what’s important to emphasize with this project is that the effects of warming combined with acidification are not the same for every generation or organism that is adapting to that environment. That’s suggested by the data and why the adaptation is limited. While within intermediate generations, organisms might be very well adapted, in later generations, the effects of warming and acidification start to behave differently on the population. That’s one of the exciting parts about the research. It’s not a static, expected result for how organisms or their populations are going to continue to grow or decay.”
For example, deMayo explains, if you took individuals in later generations that had adapted to the experimental OWA conditions and placed them into the conditions of today’s ocean, they would not fare as well.
“That’s one negative consequence, that ability to not tolerate environmental shifts is a cost and an unpredicted consequence for evolutionary adaptation in a lot of systems, not just in copepods,” says deMayo.
The researchers point out that studies looking at single stressors run the risk of making overly simplified inferences about an organism’s ability to adapt, an especially risky proposition when making conclusions about such an integral component of the food web as copepods.
“Particularly when you involve living organisms, there are complexities that you can’t predict,” says Dam. “A priori, you might make the predictions, but you have no certainty that they’re going to unfold that way. In biology these are referred to as ‘emergent properties’ or things that you cannot predict from what you know in advance and this research is a good example.”
In thinking back to the hammer comparison, Dam says impacts in the copepod population have ripple effects through the whole food web and beyond.
“If fitness decreases by say, 10%, down the road we will have a 10% decrease in population size and since these animals are the main food source for fish, a 10% decrease in the world fishery is pretty significant,” says Dam. “And this is really the best-case scenario since in the lab, they’re essentially living in hotel-like conditions so that 10% isn’t taking into consideration other factors like predation or disease. In the real world we could see fitness recovery is actually much worse.”
Additionally, Dam points out another implication is that copepods sequester CO2 and reductions in their numbers reduce the ocean’s carbon sequestration capabilities, bad news at a time when more carbon sequestration is needed.
While the research offers promise for rapid adaptation, it is a reminder that as with many things in nature there’s a catch.
“There is some welcoming news, that yes, there is a recovery of fitness but there is also sobering news that the evolutionary rescue is not complete. There’s no such thing as a free lunch,” says Dam.
44th Larval Fish Conference held virtually 24-26 June
Groton, CT 24-26 June 2021. The long awaited and anxiously prepared virtual 44th Larval Fish Conference was held, featuring more than 240 participants from 28 countries. 58 scientific talks, including 3 keynote lectures were given via Cisco’s WebEx platform, whereas networking activities such as poster presentations, ‘Meet the Speaker’ events, and Mentor hours used the innovative Gatherly platform. The technology was working out well, the preparation paid off, and delegates were overall enthusiastic about this virtual alternative, which was forced on us by Covid-19, but may have shown us new ways and concepts to broaden the societies reach and equality.
The post-conference website is housed at https://lfc44.marinesciences.uconn.edu
Special thanks go to the scientific steering committee Eric Schultz, Jacqueline Webb, and Paul Anderson. Lauren Schaller, Anne Hill, Harley Erickson, and Kate Copeland from UConn’s conference services did a great job as well preparing and running parts of the events. Support came from NOAA’s Northeast Fisheries Science Center.
Our sandlance work featured in the CapeCodFishermen
Reposted from TheCapeCodFishermen, April 28th 2021
By David N. Wiley
Bluefin tuna and striped bass crash through the waves. Seabirds wheel overhead and plunge into the water. Gape-mouthed whales rise from below. Schools of cod and dogfish hide below the surface.
While the convergence of such diverse sea life might seem accidental, those in the know thank a small, slender fish called a sand eel for the bonanza.
Also known as sand lance, these three-to-six inch forage fish are a main food source for many of the top predators in the Gulf of Maine and on Georges Bank, including some of the most commercially important species.
As their name implies, sand lance are tied to sand habitat, but not just any sand will do. To avoid predators, sand lance spend most of the night and parts of the day buried. When disturbed, they rocket out of the bottom, then dive head first and at full speed back into the sand.
As a result, their sand of choice has to be coarse enough to hold oxygen for the fish to “breathe” while buried, but soft enough to allow high-speed body penetration. One of the reasons Cape Cod is their Mecca is a band of perfect sand stretching from Stellwagen Bank along the backside of Cape Cod, past Chatham and up through Georges Bank. Whether you are a fisherman, whale watcher or seabird enthusiast, it’s this band of sand, and the sand lance that inhabit it, that makes the Cape special.
Sand and sand lance are the backbone of Stellwagen Bank National Marine Sanctuary, responsible for it being one of the top places in the United States for viewing marine life, and a centuries old, highly productive fishing ground. Yet while fishermen appreciate the importance of sand lance, little is known about their biology and most of the world does not know they exist.
To remedy the situation, a team of researchers led by scientists from Stellwagen Bank National Marine Sanctuary with partners from Boston University, Center for Coastal Studies, University of Connecticut, U.S. Geological Survey and Woods Hole Oceanographic Institution have been studying the forage fish to determine its importance and unlock some of its secrets.
One of the project’s first goals was to identify the sand lance spawning season. Using a specially designed and permitted small-mesh trawl, fished from Steve Welch’s F/V Mystic or NOAA’s R/V Auk, the team captured and examined sand lance. Thought to spawn from late fall through winter, several years of work demonstrated that sand lance on Stellwagen Bank spawn in a very narrow window at the end of November. Eggs are deposited on the seafloor and hatch after approximately six weeks.
Then things get interesting. Once hatched, sand lance are tiny, free-floating larvae for two to three months. Given this long free-floating period and the currents flowing over Stellwagen Bank, many sand lance born on the bank cannot stay there. So where do they come from and where do their offspring go?
To answer this question, the team used hydrographic modeling to backtrack to where free floating particles (like larval sand lance) would have originated prior to their sand settlement in March or April, and where drifting particles would end up two or three months after hatching.
It appears that larval sand lance settling on Stellwagen originate off the coast of Maine; years of highest sand lance abundance correspond to conditions that would have transported additional larval sand lance from as far north as Nova Scotia. The same modeling indicated that larval sand lance originating on Stellwagen Bank transport south to the Great South Channel and Nantucket Shoals (but not Georges Bank). In some years, currents moved them as far as New Jersey.
This is just another example of the interconnected world that creates a productive marine environment. Since few sand lance in the study lived past three years, the dependence on shifting currents to populate the bank could be one thing responsible for boom and bust years typical of sand lance abundance. The team is currently examining genetics of sand lance taken from throughout the Gulf of Maine, the mid-Atlantic, and eastern Canada, to gain additional insight into population structure.
Do boom-bust years influence the distribution and abundance of predators? The team investigated the association of sand lance with humpback whales and great shearwater seabirds by placing satellite tags on both species to track their movements.
Throughout the Gulf of Maine, tracking revealed that both species spend the vast majority of their time over sand lance habitat, and DNA from fecal shearwater samples showed sand lance to be the bird’s main prey. Surveys in Stellwagen also demonstrated a high co-occurrence of sand lance, humpback whales and great shearwaters.
Sand lance feed primarily from February to July, mostly on Calanus finmarchicus copepods. They stop feeding from August through October, with low levels of feeding from the end of November to January. Body growth and fat content show similar trends, with length and fat stores increasing from February to July. After July, the fish retreat to bury in the sandy bottom, conserving energy for spawning.
The team then turned its attention to the future of the valuable fish, something of extreme importance to fishermen. Ripe fish captured in November were strip-spawned on board the boats and transported to Connecticut, where eggs and larvae were raised in special tanks that allowed temperature and acidity to be manipulated to mimic future ocean conditions under climate change. Increased temperature and acidity had a dramatic negative impact on larval survival. According to Dr. Hannes Baumann, whose lab led the work, sand lance may be unusually sensitive to ocean acidification.
The future of sand lance was also a focus of team members Joel Llopiz and Justin Suca from Woods Hole Oceanographic Institution. They came to some worrisome conclusions.
The abundance of tiny C. finmarchicus copepods directly influences sand lance health: Abundant C. finmarchicus led to good parental condition and high reproductive success, while low numbers resulted in poor parental condition and poor reproductive success. Scientists have suggested climate change scenarios in the Gulf of Maine will lead to reduced abundance of this critical copepod resource. Adding to the problem was their finding that warm slope water coming through the Northeast Channel north of Georges Bank led to the death of overwintering reproductive adults.
With the Gulf of Maine warming faster than 99 percent of the world’s oceans, there is concern about the future of sand lance and its potential impact to the productivity of the Gulf of Maine, Georges Bank and other areas. While states with fisheries and other marine resources supported by sand lance cannot solve climate change issues, they can work to make sand lance more resilient to climate change. One way is to eliminate as many non-climate stressors as possible.
For example, in 2020 Massachusetts promulgated a rule limiting daily sand lance landings to 200 pounds. Rhode Island followed suit in 2021. These rules were designed to discourage the development of a commercial fishery for the species, such as the huge industrial fishery in Europe’s North Sea.
Since a commercial sand lance fishery does not currently exist here, adopting this rule by other states would be an easy, proactive way to make our waters, and the people who depend on them, more resistant to climate change disruption.
(Dr. David N. Wiley is the Research Ecologist for Stellwagen Bank National Marine Santuary. Funding for the project was provided by the Bureau of Ocean Energy Management, The Volgenau Foundation, Northeast and Woods Hole Sea Grant, International Fund for Animal Welfare, Stellwagen Bank National Marine Sanctuary and the National Marine Sanctuary Foundation. Dan Blackwood, Dr. Gavin Fay, Peter Hong, Dr. Les Kaufmann, Kevin Powers, Dr. Jooke Robbins, Dr. Tammy Silva, Mike Thompson, and Dr. Page Valentine contributed to the study)
