Dichato

Hannes returns to the US, but the experiment continues!

10 November 2025. The spring is now in full swing here in Dichato, and the days are getting steadily hotter and drier. The past 6 weeks flew by in a whirlwind of action, and with all the last minute preparation plus the vagaries of coordinated field sampling and larval rearing it definitely is with a little pride that I look back at all that we have accomplished in this short time.

We stood up the experimental setup in just a matter of days, with new tanks and in a new (better) location inside the main wet lab of the Dichato station (aka 'El Acuario'). We flew to Iquique for the October full moon and were rewarded with spawning ripe fish; and with similar ease sampled the Dichato population just two days later. A trip to the notoriously difficult Coquimbo region unfortunately ended without fish, but the sampling of the southernmost population in Puerto Montt was again timed almost perfectly for the November full moon.

All the while the setup withstood the reality test, and I taught Tamara Cuevas, a technician from the station, the many aspects large and small of the rearing methods. Tamara will now take over the daily rearing for one month, before I will return in mid December to help terminate the experiment and then take home all samples. All the best, Tamara, for your time as the main experimenter!

We are halfway through the rearing, fingers crossed that the second half will go as smoothly!

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On 9 November 2025, Hannes stands at the 14C tank of the common garden experiment

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Tamara Cuevas preserving Chilean silverside larvae in ethanol

The Chilean silverside experiment begins to produce data!

 

29 October 2025. While the fish from Dichato in the coldest treatment have just begun to hatch, their conspecifics from Iquique and Dichato growing at the warmest temperatures have already more than doubled their size at hatch and are therefore ready for a first length sample. This is always a particularly sweet and rewarding moment, when after all the work to get to this point - it now finally arrived.

In each rearing container, 80 fish remain to grow further, while the rest is sampled, preserved in ethanol and then measured via calibrated pictures. Then the size difference between hatch and the first time point is divided by the number of days to obtain our first growth rate estimates of the second experiment!

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On 29 October, the embryos from Dichato are about to hatch in the 14C treatment

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Newly hatched larvae from the Iquique population (20C treatment)

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10 day old larvae from the 23C treatment, photographed on a gridded background to later measure them digitally

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On 20 October, Tamara Cuevas helps distribute newly hatched larvae into replicate rearing containers

Chilean silversides are spawning in Iquique and Dichato

7 October 2025. Its been a truly wild last few days. In Dichato, we were racing against time to get all the setup ready, including an emergency bus trip overnight to Santiago to retrieve the missing chillers from a central aquarium store, and a Saturday morning frenzied work session to get everything up and running before the first sampling trip to the north. On Saturday night Hannes flew to the desert city of Iquique at 20S, a place of unreal beauty and harsh landscapes of sand, rocks, and ocean.

Thanks to the incredible help and expertise from local collaborators Prof. Miguel Araya and Dr. Cristian Asocars, the Sunday (5 Oct) morning  fishing via gill net was almost too good to be true. With just one cast, we caught upwards of 400 Chilean silversides, all running ripe - perhaps inspired as we often found for northern hemisphere silversides - by the full moon. The fertilization in the boat and then transport of the embryos back to the lab via airplane all went without problems - a rare moment to cherish as an experimenter.

The kicker? The same full moon also appeared to entice the silversides in Dichato, and so a spontaneous fisherman went out with Hannes Tuesday night to - lo and behold - again catch all the fish, all running ripe, we needed to start the second population of the experiment.

48 hours. 2 populations more than thousand miles apart sampled and brought as embryos into the common garden experiment. Maybe I should consider a generous offering to San Pedro, the saint of the fishermen whose statue is in everything fishing harbor in Chile.

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The day of fertilization, the cell division in the fertilized embryos is beginning.

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Cristian Azocar recovering the gill net full of spawning ripe silversides

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On 6 October 2025, Hannes gave a spontaneous talk in front of students and faculty of the Marine Science Department of the University Arturo Prat (UNAP) in Iquique