Imagery

[Research news] Live staining of silverside neuromasts at URI

Posted on July 27, 2018July 27, 2018 by Hannes Baumann

By Emma Cross.

23 Juli 2018. Yesterday Hannes and Emma took a short road trip to the University of Rhode Island to visit Professor Jacqueline Webb’s lab to learn about in vivo fluorescent imaging.

This technique involves placing live fish in a fluorescent mitrochondrial stain for 5 minutes before imaging different areas of the fish under a dissecting microscope equipped with an epiflourescence light source. This allowed us to visualize small sense organs called neuromasts located in tubular canals in the head, trunk and tail, which form the fish sensory lateral line system used to detect water flows.

We are interested to see if high CO₂ conditions affect these neuromasts in the Atlantic silverside, which could impact their critical schooling behaviors.

Take a look at some of the stunning images below! Thank you to Jackie and all the lab for a fun-filled day!

[Lab news] Baumann lab attends the Larval Fish Conference in Victoria

Posted on July 2, 2018July 2, 2018 by Hannes Baumann

Holding the fort at the Rankin lab were Emma and Sydney, who did an excellent job. Thank you guys!

Victoria08 — The Delta Victoria Ocean Pointe Resort was the conference venue

Victoria10 — Before the conference, we all attended a workshop on larval fish identification

https://www.fishersci.com/shop/products/fisherbrand-class-a-clear-glass-threaded-vials-attached-caps-pe-poly-seal-cone-liner-8/14955326 — Whale-watching with Corinne, Julie & Chris

Here is how Julie experienced her first LFC:

Ever since attending the American Fisheries Society conference in 2014, I’ve wanted to go to another fish-focused conference. I was lucky enough to attend the 42nd annual Larval Fish Conference this year in Victoria, British Columbia, and it surpassed all my expectations. The week started off with a larval fish identification workshop where we got to learn techniques from renowned larval fish experts (and see some really cool fish larvae!). The talks were impressive and thought-provoking, providing many new ideas for research and how to give an engaging talk. My favorite part was meeting all the larval fish ecologists whose publications I’ve been reading for my thesis. I spent most of my evenings exploring Victoria with other grad students attending the conference and left with many new friends from institutes all over the world! The trip ended with a whale watch, where we saw a pod of 5 Orcas. Overall, the Larval Fish Conference was a great experience that I hope to someday attend again!

Oral presentations:

Pringle, J. and Baumann, H. Sex-specific growth and mortality patterns in juvenile Atlantic silversides (Menidia menidia) from Connecticut waters. Talk. 42nd Larval Fish Conference, Victoria, BC, Canada 24-28 June 2018

Murray, C.S., Wiley, D., and Baumann, H. Early life stages of the northern sand lance Ammodytes dubius show high sensitivity to acidification and warming in a CO₂ × temperature factorial experiment. Talk. 42nd Larval Fish Conference, Victoria, BC, Canada 24-28 June 2018

Victoria04 — Harbor front with Parliament building

Victoria06 — Local celebrity, the one eyed seal

[Lab video] How a new silverside experiment starts

Posted on July 2, 2018 by Hannes Baumann

29 June 2018. A new experiment with Atlantic silversides (Menidia menidia) starts and as usual, it’s an all hand on deck operation. This time, we have Chris Tsang shadowing all of us and Emma professionally explaining the process.

Have a look for yourself!

[Lab news] Video of Mumford Cove probe swap

Posted on June 14, 2018June 14, 2018 by Hannes Baumann

14 June 2018. Members of the Baumann and Mason lab went on a trip to Mumford Cove, today, and Chris Tsang went along with his GoPro. Thanks to Charlie, the skipper, the ride was smooth and a pleasure, a swapping our pH, Temperature, oxygen, and salinity sensor was successfully swapped with a new one recording for the next weeks in 30 minute intervals. Wes Hoffman from the Mason lab, collected zooplankton with a Bongo-net. Sydney Stark, our NSF-REU student this summer, came along just for the fun.

See the fun for yourself!

[Research news] A day at Harvards MCZ

Posted on June 9, 2018June 9, 2018 by Hannes Baumann

Friday, 8 June 2018. Hannes and Maria traveled to Boston’s Harvard University to meet with Valentina di Santo from the Lauder Lab at the Museum for Comparative Zoology. Thanks to our collaborators there, we were able to use a 2D-digital X-ray machine there, which we needed to complete the next big step in our Menidia Gene project.

Vials — Genetic & body samples went in different vials

Maria-vials — Maria Akopyan processing the fish after x-raying

Maria-Valentina — Maria and Valentina in the shark section of the collection

A few weeks ago, Maria had already measured each individual fish's length, weight, shape, routine metabolism, and maximum sustained swim speed. The next trait we're keen on mapping quantitatively to the silverside genome is the number of vertebrae, which we know increases in wild populations from south to north. What will our South/North hybrid F2 generation show?

Lunch-with-George-Lauder-and-lab — At the Lauder lab, ‘lunch together’ is common thing

Hannes-Latimeria — The famous Latimeria from the collection

Thanks to Valentina's excellent help, the work went without a hitch. At the end, we even had some spare time to enjoy the great atmosphere int the Lauder Lab during lunchtime, the tour through various lab installations, the experimental fish, and even the adjacent Harvard Zoological museum. Thank you all for the fun day at Harvard!

x-ray-settings — Settings used for x-raying juvenile silversides

Hannes-Valentina — Hannes and Valentina in the x-ray room

George-Lauder-swim-lab — George Lauder adjusting equipment in the swim lab

The 282 fish are now split in a DNA sample for extraction and a body sample for further trait measurements.

A busy day in the Baumann Lab

Posted on May 19, 2018May 22, 2018 by Hannes Baumann

On 18 May 2018, the Baumann lab teemed with activity. Maria Akopyan from Cornell University was busy phenotyping juvenile silversides for our Menidia Gene project. Mia and Mackenzie were busy working up field samples of silversides. And Hannes prepared adult silverside samples for later analyses.

[Funding] UConn Today announces new NSF-silverside project

Posted on April 21, 2018April 21, 2018 by Hannes Baumann

Click on the link or the image blow to access the article in UConn Today …

Project page | Collaborators

[New publication] No CO2 effects on silverside starvation

Posted on April 1, 2018April 1, 2018 by Hannes Baumann

31 March 2018. We’re happy to announce that Marine Biology just published our latest study examining the starvation tolerance of silverside larvae and juveniles at contrasting CO₂ conditions. We compiled observations from five separate experiments spanning different years, laboratories, temperatures, life stages, and CO₂ levels. Contrary to expectation, we found that starvation rates were largely independent of the CO₂ environment in this fish species.

One major set of data was produced by Elle Parks as part of her Research Experience for Undergraduates (NSF-REU) in summer 2017. Well done, everybody!

Baumann, H., Parks, E.M.*, and Murray, C.S.* (2018)

Starvation rates in larval and juvenile Atlantic silversides (Menidia menidia) are unaffected by high CO₂ conditions.
Marine Biology 165:75-83

BaumannLab170609b285s — Hannes shows Elle Parks (REU 2017), how individual screen with enumerated embryos are suspended into the replicate rearing containers. (Photo: Peter Morenus, UConn)

BaumannLab170609b046s — On 9 June, Elle and Julie strip-spawn Atlantic silverside females into spawning dishes covered in window screen for eggs to attach. (Photo: Peter Morenus, UConn)

Starvation-figure-MABI2018 — M. menidia. (A) Relative cumulative starvation mortalities of early juveniles reared under ambient (grey line, diamonds) vs. high CO₂ conditions (black line, circles). Symbols depict individual replicates, lines represent treatment means. (B) Total length of juveniles perishing during the experiment at ambient (grey diamonds) vs. high CO₂ conditions (black circles). Lines represent the median(solid lines), 5th and 95th percentiles (dashed lines) of TL estimated with locally weighted, non-parametric density estimators. The initial TL distribution at the beginning of the experiment is depicted on day 0 as the median (white circle), 5th/95th percentiles (whiskers) and the minimum and maximum (white stars).

[Research news] Silversides in a CT scanner

Posted on March 27, 2018March 27, 2018 by Hannes Baumann

26 March 2018. Today we got our first glimpse of an incredible new way of imaging the inner calcified structures of a fish body, particularly the ear bones (otoliths), of which every teleost fish has six, three on each side inside the skull. Otoliths are long known to fish ecologists for their properties to record and store information about a fish’s age, growth and habitat. With an adult Atlantic silverside, Hannes visited John Shepherd, facilities scientist and member of the Goldhamer lab at UConn, Storrs (Biology Physics Building), who showed us the use of a new, state-of-the-art micro CT-scanner (IVIS). Turns out, the system effortlessly imaged all six otoliths inside of the fish’s head. Later in the year, we will use the technique to image silversides reared at contrasting CO₂ conditions to see whether they differ in their otolith size, volume, and structure. Thank you, John, for this truly inspiring demonstration!

[Research feature] Our multistressor NSF project in the spotlight

Posted on February 21, 2018April 5, 2025 by Hannes Baumann

This research feature makes the case for multistressor research to a broad general audience and introduces our NSF project and its participants. Download the feature by clicking on the pictures or the link below.

Download the Research feature